What technique is utilized in non-viral gene therapy to temporarily open pores in the cell membrane?
Answer
Electroporation
While scientists explore non-viral methods to avoid issues associated with viruses, these methods rely on different physical or chemical mechanisms to introduce genetic material. Electroporation is specifically categorized as a physical method within this group. It involves applying brief, controlled electrical pulses to the target cells. These pulses induce transient changes in the cell membrane structure, creating temporary openings or pores through which the therapeutic DNA can enter the cell nucleus, bypassing the need for a biological virus envelope.
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