What is considered the gold standard microscopic confirmation technique for diagnosing a mycosis?
Answer
Potassium hydroxide (KOH) slide examination
For definitive identification of a fungal infection, direct microscopic visualization of the causative agent is necessary. The gold standard procedure involves a provider gently scraping a small amount of skin scale from the lesion onto a glass slide. This sample is then treated with potassium hydroxide (KOH), which works to dissolve human skin cells while leaving the fungal elements intact. Subsequent examination under a microscope allows for the direct viewing and confirmation of fungal hyphae or spores, confirming the presence of mycosis.
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